Flow Imaging – Popular Techniques and Benefits for Cell Research 


Flow imaging in cell research refers to techniques used to study and analyze the flow properties of cells in a fluidic environment. These techniques allow researchers to investigate various aspects of cellular behavior, including cell migration, adhesion, deformability, and interactions with other cells or biomolecules. These techniques provide quantitative data and visual insights into cell behavior, allowing researchers to understand cellular processes, study disease mechanisms, develop diagnostics, and explore therapeutic approaches. Flow imaging in cell research contributes to fields such as immunology, cancer biology, tissue engineering, and regenerative medicine.

Common Flow Imaging Techniques Used in Cell Research

Microfluidic Cell Sorting: Microfluidic devices with imaging capabilities can sort cells based on their flow properties. By flowing a suspension of cells through microchannels, cameras or optical sensors capture images of individual cells, and algorithms analyze parameters like size, shape, and fluorescence signals to sort cells into different populations.

Live Cell Imaging and Tracking: Fluorescence microscopy combined with time-lapse imaging allows researchers to observe and track individual cells as they flow in real-time. This technique provides valuable information about cell behavior, motility, and interactions with other cells or substrates.

Cell Deformability Analysis: Techniques such as deformability cytometry or microfluidic stretching assays measure the mechanical properties and deformability of cells as they flow through microchannels or encounter controlled forces. These methods can provide insights into cell stiffness, elasticity, and their ability to navigate the narrow capillaries or tissues.

Cell Migration and ChemotaxisFlow imaging techniques can be employed to investigate cell migration in response to chemical gradients or fluidic forces. Researchers can use microfluidic devices with precisely controlled flow patterns or concentration gradients to study how cells respond to different stimuli and navigate within complex environments.

Key Advantages of Flow Imaging Studies

Real-Time Analysis: Flow imaging allows researchers to study cell behavior in real time. By capturing images or videos of cells as they flow, researchers can observe dynamic processes such as cell migration, adhesion, and interactions with high temporal resolution. It enables the monitoring of cellular events and provides insights into the kinetics and timing of various cellular processes.

Single-Cell Analysis: Single-cell analysis is valuable for understanding cellular heterogeneity, investigating rare cell types, and identifying specific cellular responses or phenotypes within a population. According to Cell, imaging flow cytometry combines the multiparameter, high-throughput capabilities of conventional flow cytometry with spatial information at a resolution of a single cell.

Quantitative Measurements: Flow imaging techniques provide quantitative data about cell properties and behaviors. Researchers can extract measurements such as cell velocity, displacement, deformation, adhesion strength, and other parameters by analyzing images or videos. These quantitative measurements enable the characterization and comparison of cell populations, facilitating statistical analyses and objective evaluation of experimental outcomes.

Controlled Experimental Conditions: Flow imaging techniques can be combined with microfluidic devices that offer precise control over experimental conditions! The ability to create controlled microenvironments enhances the reproducibility and reliability of experiments.


Flow imaging techniques provide valuable tools for studying cell behavior, cellular responses to stimuli, and the underlying mechanisms in a controlled and quantitative manner. These techniques contribute to advancements in cell biology, tissue engineering, drug discovery, and disease research.